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ucsb_beckman_echo [2026/06/23 02:34] – created alexandraucsb_beckman_echo [2026/09/30 22:09] (current) – alexandra
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 **About**\\ **About**\\
 The Echo 525 Acoustic Liquid Handler is a non-contact, acoustic droplet ejection (ADE) instrument capable of transferring nanoliter to microliter volumes of liquid with high precision and minimal waste. Unlike traditional pipette-based liquid handlers, the Echo 525 uses focused acoustic energy to eject droplets as small as 2.5 nL directly from a source plate to a destination plate without tips. This eliminates cross-contamination, reduces reagent consumption, and enables highly flexible compound and assay dispensing. It is compatible with a wide range of plate formats (384-well, 1536-well) and is controlled by the **Echo Liquid Handler Software**. The Echo 525 Acoustic Liquid Handler is a non-contact, acoustic droplet ejection (ADE) instrument capable of transferring nanoliter to microliter volumes of liquid with high precision and minimal waste. Unlike traditional pipette-based liquid handlers, the Echo 525 uses focused acoustic energy to eject droplets as small as 2.5 nL directly from a source plate to a destination plate without tips. This eliminates cross-contamination, reduces reagent consumption, and enables highly flexible compound and assay dispensing. It is compatible with a wide range of plate formats (384-well, 1536-well) and is controlled by the **Echo Liquid Handler Software**.
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-**Equipment Reservation** 
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 **User Notes** **User Notes**
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   * Wipe off and decontaminate equipment surfaces and source/destination plates with 70% ethanol before and after use. **Do not use bleach.**   * Wipe off and decontaminate equipment surfaces and source/destination plates with 70% ethanol before and after use. **Do not use bleach.**
   * Ensure source plates are filled to the recommended minimum volume to allow acoustic ejection (typically ≥ 6 µL per well for 384-well LDV plates).   * Ensure source plates are filled to the recommended minimum volume to allow acoustic ejection (typically ≥ 6 µL per well for 384-well LDV plates).
-  * Inspect source plates for bubbles or particulates before loading — these can interfere with droplet ejection. +  * Inspect source plates for bubbles or particulates before loading as these can interfere with droplet ejection.
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-====== Echo Liquid Handler Software ====== +
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-**About**\\ +
-The Echo is controlled using the **Echo Liquid Handler Software** (also compatible with **Labcyte/Beckman Echo Survey Software** and **Cherry Picking** workflows). The software provides a graphical interface to define source and destination plate layouts, set transfer volumes, and execute transfers. It supports survey mode (to assess fluid levels in source plates) and transfer mode. Protocols can be saved as reusable scripts and imported/exported in CSV format for easy integration with external workflows. +
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-**User Notes** +
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-  * Always run a **fluid survey** of source plates before performing transfers to verify adequate fluid levels and detect any problematic wells. +
-  * Make sure to **save your transfer protocol** before running as unsaved protocols will be lost if the software closes unexpectedly. +
-  * Review the transfer report generated after each run to confirm all transfers completed successfully.+
  
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 ====== Reference Documentation ====== ====== Reference Documentation ======
 +User manual: {{ :echo_525_manual.pdf |}}\\
 +Consumables: {{ :echo_acoustic_liquid_handler_consumables_brochure.pdf |}}\\
 +Plate audit applications: {{ :plate_audit.pdf |}}
  
-**Echo 525**\\ 
-[[https://|echo525_operatingmanual.pdf]]\\ 
-[[https://|echo525_quickstartguide.pdf]]\\ 
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-**Echo Software**\\ 
-[[https://|echo_software_userguide.pdf]]\\ 
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-**Application Notes**\\ 
-[[https://|echo_assay_development_guide.pdf]]\\ 
-[[https://|echo_compound_management_guide.pdf]]\\ 
ucsb_beckman_echo.1782182050.txt.gz · Last modified: by alexandra