About
The Agilent BioTek Cytation C10 is a high-content confocal imaging reader that combines automated spinning-disk confocal and widefield fluorescence microscopy with conventional multimode microplate reading in a single, compact benchtop instrument. Its proprietary spinning-disk confocal module delivers exceptional optical sectioning and resolution, enabling deeper imaging into thick biological samples such as 3D cell cultures and whole organisms with improved clarity and reduced out-of-focus blur. High-quality optical components — including a Hamamatsu sCMOS Orca camera, Olympus objectives, Semrock filters, and laser-based illumination — enable publication-quality imaging. Automated water-immersion objectives capture more light with shorter exposure times, reducing phototoxic impact on live cells. Onboard environmental controls (incubation, CO₂/O₂) support live cell assays, and multiplate live cell experiments can be run via integration with the Agilent BioTek BioSpa 8 automated incubator. The system is controlled with Agilent BioTek Gen5 software for imaging acquisition and analysis.

User Notes

Imaging Modes

• Confocal fluorescence
• Widefield fluorescence
• Phase contrast
• Brightfield
• Color brightfield
• High-contrast brightfield

Imaging Methods

• Single-color and multi-color acquisition
• Z-stacking (optical sectioning)
• Time-lapse imaging
• Montage (large area tiling)

Magnification Range

• 1.25× to 60× (air objectives)
• 4× to 40× (phase contrast)
• 20× to 60× (confocal)
• 40× and 60× (water immersion)