====== BioTek Cytation C10 Confocal Imaging Reader ======

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**About**\\
The Agilent BioTek Cytation C10 is a high-content confocal imaging reader that combines automated spinning-disk confocal and widefield fluorescence microscopy with conventional multimode microplate reading in a single, compact benchtop instrument. Its proprietary spinning-disk confocal module delivers exceptional optical sectioning and resolution, enabling deeper imaging into thick biological samples such as 3D cell cultures and whole organisms with improved clarity and reduced out-of-focus blur. High-quality optical components — including a Hamamatsu sCMOS Orca camera, Olympus objectives, Semrock filters, and laser-based illumination — enable publication-quality imaging. Automated water-immersion objectives capture more light with shorter exposure times, reducing phototoxic impact on live cells. Onboard environmental controls (incubation, CO₂/O₂) support live cell assays, and multiplate live cell experiments can be run via integration with the **Agilent BioTek BioSpa 8** automated incubator. The system is controlled with **Agilent BioTek Gen5** software for imaging acquisition and analysis.

**User Notes**

====== Imaging Modes and Capabilities ======

**Imaging Modes**

  * Confocal fluorescence
  * Widefield fluorescence
  * Phase contrast
  * Brightfield
  * Color brightfield
  * High-contrast brightfield

**Imaging Methods**

  * Single-color and multi-color acquisition
  * Z-stacking (optical sectioning)
  * Time-lapse imaging
  * Montage (large area tiling)

**Magnification Range**

  * 1.25× to 60× (air objectives)
  * 4× to 40× (phase contrast)
  * 20× to 60× (confocal)
  * 40× and 60× (water immersion)

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