Differences

This shows you the differences between two versions of the page.

--- ucsb_aligent_cytation_imager [2026/07/08 02:03] – created alexandra
+++ ucsb_aligent_cytation_imager [2026/09/17 22:18] (current) – alexandra
@@ Line 1: / Line 1: @@
-====== Agilent Cytation 10 Confocal Imager ======
+====== BioTek Cytation C10 Confocal Imaging Reader ======
 {{:screenshot_2026-07-07_at_7.02.54 pm.png?300|}}
 **About**\\
+The Agilent BioTek Cytation C10 is a high-content confocal imaging reader that combines automated spinning-disk confocal and widefield fluorescence microscopy with conventional multimode microplate reading in a single, compact benchtop instrument. Its proprietary spinning-disk confocal module delivers exceptional optical sectioning and resolution, enabling deeper imaging into thick biological samples such as 3D cell cultures and whole organisms with improved clarity and reduced out-of-focus blur. High-quality optical components — including a Hamamatsu sCMOS Orca camera, Olympus objectives, Semrock filters, and laser-based illumination — enable publication-quality imaging. Automated water-immersion objectives capture more light with shorter exposure times, reducing phototoxic impact on live cells. Onboard environmental controls (incubation, CO₂/O₂) support live cell assays, and multiplate live cell experiments can be run via integration with the **Agilent BioTek BioSpa 8** automated incubator. The system is controlled with **Agilent BioTek Gen5** software for imaging acquisition and analysis.
+**User Notes**
+====== Imaging Modes and Capabilities ======
+**Imaging Modes**
+  * Confocal fluorescence
+  * Widefield fluorescence
+  * Phase contrast
+  * Brightfield
+  * Color brightfield
+  * High-contrast brightfield
+**Imaging Methods**
+  * Single-color and multi-color acquisition
+  * Z-stacking (optical sectioning)
+  * Time-lapse imaging
+  * Montage (large area tiling)
+**Magnification Range**
+  * 1.25× to 60× (air objectives)
+  * 4× to 40× (phase contrast)
+  * 20× to 60× (confocal)
+  * 40× and 60× (water immersion)
+----